Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen

Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen

Blog Article

The establishment of protocols read more to differentiate human pluripotent stem cells (hPSCs) including embryonic (ESC) and induced pluripotent (iPSC) stem cells into functional hepatocyte-like cells (HLCs) creates new opportunities to study liver metabolism, genetic diseases and infection of hepatotropic viruses (hepatitis B and C viruses) in the context of specific genetic background.While supporting efficient differentiation to HLCs, the published protocols are limited in terms of differentiation into fully mature hepatocytes and in a smaller-well format.This limitation handicaps the application of these cells to high-throughput assays.Here we describe a protocol allowing efficient and consistent hepatic Hoodie differentiation of hPSCs in 384-well plates into functional hepatocyte-like cells, which remain differentiated for more than 3 weeks.

This protocol affords the unique opportunity to miniaturize the hPSC-based differentiation technology and facilitates screening for molecules in modulating liver differentiation, metabolism, genetic network, and response to infection or other external stimuli.